The squad selection comes after the recent closure of the Ghanaian transfer window.
The squad includes eight forwards, and among them is Cameroonian forward Albert Eonde, who joined the club in August and has impressed during training sessions.
Richmond Ayi, Richard Attah and Eric Ofori Antwi have been named as the goalkeepers, with new signing Michael Ampadu included among the full-backs.
Meanwhile, Gladson Awako has left out of the squad for the upcoming season.
Having finished 12th last season, Hearts of Oak will hope to improve their performance under Dutchman coach Martin Koopman.
The Phobians will be looking for a solid start to the season as they face Real Tamale United in their first game on September 15.
Full squad below:
Eric Ofori Antwi
Thomas Qwaku Nsoh
Kelvin Osei Assibey
Glid Jeodan Otanga
Abdul Aziz Nuurudeen
Yaw Amankwa Baafi
Gideon Asante Yeboah
Kwadwo Obeng Jnr
Kashala Ramos Wanet